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dsacas9 krab lentiviral expression plasmid  (Addgene inc)


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    Structured Review

    Addgene inc dsacas9 krab lentiviral expression plasmid
    Dsacas9 Krab Lentiviral Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15695 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsacas9/psPAX2+(Plasmid+%2312260)/us12428631-1135-14-25
    Average 98 stars, based on 15695 article reviews
    dsacas9 krab lentiviral expression plasmid - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Clone Assay:

    Article Title: How chromosomal translocations arise to cause cancer: gene proximity, trans-splicing and DNA end joining
    Article Snippet: In addition, a U6 promoter driven guide RNA/scaffold cassette was cloned from the AddGene plasmid #60958 for dSaCas9, or from the AddGene plasmid #42230 for the deSpCas9 protein, to create two pairs of vectors that can be used for the establishment of stable cell lines. .. In addition, a U6 promoter driven guide RNA/scaffold cassette was cloned from the AddGene plasmid #60958 for dSaCas9, or from the AddGene plasmid #42230 for the deSpCas9 protein, to create two pairs of vectors that can be used for the establishment of stable cell lines. ..

    Plasmid Preparation:

    Article Title: How chromosomal translocations arise to cause cancer: gene proximity, trans-splicing and DNA end joining
    Article Snippet: In addition, a U6 promoter driven guide RNA/scaffold cassette was cloned from the AddGene plasmid #60958 for dSaCas9, or from the AddGene plasmid #42230 for the deSpCas9 protein, to create two pairs of vectors that can be used for the establishment of stable cell lines. .. In addition, a U6 promoter driven guide RNA/scaffold cassette was cloned from the AddGene plasmid #60958 for dSaCas9, or from the AddGene plasmid #42230 for the deSpCas9 protein, to create two pairs of vectors that can be used for the establishment of stable cell lines. ..

    Article Title: Engineered Sdd7 cytosine base editors with enhanced specificity
    Article Snippet: Cord blood samples were obtained from the Seoul National University Hospital AllCord Bank for research purposes. .. The plasmids pCMV_BE4max (Addgene plasmid #112093), dSaCas9 (Addgene plasmid #138162), and pCMV-MMLVgag-3 × NES-ABE8e (Addgene plasmid #181751), YE1-BE4max (Addgene plasmid #138155), YE2-BE4max (Addgene plasmid #138156), p2T-CMV-eA3Amax-BlastR (Addgene plasmid #152997), and SpCas9 TadCBEd (Addgene plasmid #193835) were a gift from David Liu. .. To construct the Sdd7-based base editors, Sdd7 double strand DNA fragment was inserted into the pCMV_BE4max vector in place of the APOBEC domain using Gibson Assembly (New England Biolabs).

    Article Title: Engineered Sdd7 cytosine base editors with enhanced specificity.
    Article Snippet: .. The plasmids pCMV_BE4max (Addgene plasmid #112093), dSaCas9 (Addgene plasmid #138162), and pCMV-MMLVgag-3 ×NES-ABE8e (Addgene plasmid #181751), YE1-BE4max (Addgene plasmid #138155), YE2-BE4max (Addgene plasmid #138156), p2T-CMV-eA3Amax-BlastR (Addgene plasmid #152997), and SpCas9 TadCBEd (Addgene plasmid #193835) were a gift from David Liu. .. To construct the Sdd7-based base editors, Sdd7 double strand DNA fragment was inserted into the pCMV_BE4max vector in place of the APOBEC domain using Gibson Assembly (New England Biolabs).

    Article Title: Efficient DNA base editing via an optimized DYW-like deaminase
    Article Snippet: .. dsaCas9 (Addgene plasmid #138162) and their gRNAs were used in orthogonal R-loop assay ( ). ..

    Stable Transfection:

    Article Title: How chromosomal translocations arise to cause cancer: gene proximity, trans-splicing and DNA end joining
    Article Snippet: In addition, a U6 promoter driven guide RNA/scaffold cassette was cloned from the AddGene plasmid #60958 for dSaCas9, or from the AddGene plasmid #42230 for the deSpCas9 protein, to create two pairs of vectors that can be used for the establishment of stable cell lines. .. In addition, a U6 promoter driven guide RNA/scaffold cassette was cloned from the AddGene plasmid #60958 for dSaCas9, or from the AddGene plasmid #42230 for the deSpCas9 protein, to create two pairs of vectors that can be used for the establishment of stable cell lines. ..

    other:

    Article Title: High-efficiency base editing for nuclear and mitochondrial DNA with an optimized DYW-like deaminase.
    Article Snippet: The reaction mixture was incubated at 37◦C for 8 h. After deamination, the genomic DNA was purified using the DNeasy Tissue Kit (Qiagen), and RNase A (50 μg/mL) was added for the elimination of gRNA.

    Article Title: Orthogonal transcriptional modulation and gene editing using multiple CRISPR/Cas systems.
    Article Snippet: 528 dSpCas9-SunTag_10x amplified from Addgene plasmid 107310. dSV40-NLS-dCas9-HA-NLS-529 NLS-10xGCN4 was a gift from Hui Yang (Addgene plasmid # 107310 ; 530 http://n2t.net/addgene:107310 ; RRID:Addgene_107310).



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